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Image Search Results
Journal: Oncogene
Article Title: Microenvironment-induced downregulation of miR-193b drives ovarian cancer metastasis
doi: 10.1038/onc.2015.43
Figure Lengend Snippet: ( a ) Ex vivo omental assay. HeyA8 cells stably expressing GFP and miR-193b or control vector were seeded on pieces of full, freshly removed, human omentum and cultured ex vivo for 7 days. The growth of GFP-expressing cells overexpressing miR-193b or control vector on the pieces of omentum were imaged with a fluorescent microscope ( top ) and quantified on a fluorescent plate reader after enzymatic digestion of the tissue ( right ). Representative images of Hematoxylin and Eosin staining of omental sections ( bottom )(mean±SD; 2 independent experiments with 3 replicates) ( b ) Xenograft mouse model of metastasis. Female athymic nude mice were injected i.p. with 1×10 6 HeyA8 cells stably expressing miR-193b or the vector control (10 mice per group). Mice were euthanized after 15 days and the tumors surgically resected and weighed. Left: Quantification of the tumor weight of HeyA8 tumors stably expressing miR-193b compared to control vector. The mean tumor weights are indicated and marked by dashes. Right: The tumor sections (n=3) were stained for Ki-67 and cleaved (Cl.) caspase 3 expression to test for proliferation and apoptosis, respectively. ( c ) HeyA8 cells (1×10 6 ) stably expressing miR-193b inhibitor miR-Zip-193b(Anti-miR-193b) or control vector were injected i.p. in female athymic nude mice (n=10/group). Mice were euthanized after 15 days and the tumors surgically resected and weighed. Quantification of the tumor weight of HeyA8 tumors stably expressing miR-Zip-193b(Anti-miR-193b) compared to control vector is plotted. The mean tumor weights are indicated and marked by dashes. * p<0.01, Students t test.
Article Snippet: At the end point the cancer cells colonizing the omentum were imaged with an
Techniques: Ex Vivo, Stable Transfection, Expressing, Control, Plasmid Preparation, Cell Culture, Microscopy, Staining, Injection